DNSH what is it, and why should you be testing for it?

DNSH (3.5-dinitro-N’-(5- nitrofurfurylidene) salicylic acid hydrazide); has been widely used as a food additive for the treatment of gastrointestinal infections (caused by Escherichia coli and Salmonella) in pig, poultry, and aquatic production. However, DNSH has recently been prohibited for use as a feed additive for its potential carcinogenicity of the parent molecule and its metabolites in humans. DNSH clears rapidly from the animal body within a few days post ingestion, but its toxic metabolites remain for a longer period. Detection of DNSH and its toxic metabolites should be implemented for the regulation and control of these substances since DNSH is banned as a feed additive.

Nitrofurans are synthetic broad-spectrum antimicrobial agents which are rapidly absorbed and extensively bio-transformed, giving rise to protein-bound residues. The 3,5-dinitrosalicyclic acid hydrazide (i.e. DNSH) metabolite is readily transformed from the parent nifursol. The metabolites are routinely used as marker residues for control of the prohibited treatment of food-producing animals with nitrofurans in the European Union (EU).

In order to help the industry comply with EU commission Regulation (EU) 2019/1871 that has made it mandatory to test for DNSH Randox Food Diagnostics have created the DNSH ELISA.

This ELISA provides a fast, reliable, analytical method to detect the presence of DNSH in a test sample of prawn/shrimp. If performing the test using duplicate wells, this kit has the ability to analyse 40 samples per plate. Randox Food also offer ELISA kits for the following Nitrofurans; AOZ, AMOZ, AHD and SEM.

For more information email us at: info@randoxfooddiagnostics.com